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Kinetic parameters for GiACS1 and GiACS21.
| Enzyme | Substrate | K′ or Km (μM) | Vmax (μmol/mg/min) | h2 | Ki of triacsin C (μM) |
|---|---|---|---|---|---|
| GiACS1 | Palmitic acid | 13.09 ± 2.42 | 1.25 ± 0.12 | 1.36 | 0.18 |
| ATP | 279 ± 18.46 | 2.19 ± 0.04 | |||
| GiACS2 | Palmitic acid | 11.15 ± 0.54 | 2.64 ± 0.06 | 2.19 | 0.23 |
| ATP | 175 ± 18.68 | 3.55 ± 0.08 |
1Values for K′, Km, and Vmax are expressed as mean ± SD; 2h = Hill coefficient.
