Fig 1. Glutamine uptake is increased by latent KSHV infection.

(A) Intracellular glutamine levels are elevated following KSHV infection. Box and whisker plot showing relative level of intracellular glutamine determined in a metabolomics screen of Mock- (white) and KSHV-infected (grey) TIME cells at 48 and 96 hpi[8]. The average intensity of intracellular glutamine from six biological replicates is denoted by (+) sign. P-value < 0.0001 at both time points. (B) Glutamine uptake is increased during latent KSHV-infection. Ninety-six hpi, Mock- and KSHV-infected TIME cells were incubated with [³H]-Glutamine for 10 min, followed by intracellular quantification of radioactivity, normalized to total protein. Error bars represent the SEM of three independent experiments, p-value < 0.05.