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. 2015 Jul 22;5:11379. doi: 10.1038/srep11379

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Snapshots from movie. A. Upper panels: control embryos injected with water solution. Bottom panels: 100 mM of EDTA injected extracellularly at the end of cellularization and embryos imaged shortly after injection. Mesoderm invagination and further development failed in EDTA injected embyos. Calcium concentration is reported by CaGr. B. Quantification of the number of spikes produced during gastrulation in the embryos injected with different concentrations of EDTA. Mean values and standard deviations are shown on the graph. P-value of ANOVA test is p < 0.0001.