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. 2015 Jul 22;5:12361. doi: 10.1038/srep12361

Figure 4. Combination index and dose-dependent inhibition of LPS-induced inflammatory indicator production by single active compounds or compound combinations.

Figure 4

RAW264.7 macrophage cells were pretreated with different concentrations of tested samples in the presence of 1 μg/ml LPS for 24 h. Dose-effect curves of PA, TN, and their combinations in inhibition of (a) NO and (b) IL-6 production. Dose-effect curves of TN at higher concentrations in inhibition of (c) NO and (d) IL-6 production. Dose-effect curves of 10 phenolic acids in inhibition of (e) NO and (f) IL-6 production. Dose-effect curves of 4 tanshinones in inhibition of (g) NO and (h) IL-6 production. (i) Combination index. The combination index was calculated using CompuSyn software based on the data of 3 replicates. 0.9 < CI < 1.1 indicated an additive effect. CP: Cardiotonic Pill; PA: combination of 10 phenolic acids; TN: combination of 4 tanshinones; PA&TN: combination of PA and TN.