Table 1. IC50 and activity contribution of 10 phenolic acids and 4 tanshinones.
| No. | Compound | NO |
IL-6 |
||||
|---|---|---|---|---|---|---|---|
| C (μM) | C50 (μM) | ACI50 (%) | C (μM) | C50 (μM) | ACI50 (%) | ||
| P1 | Danshensu | 22.21 | 123.2 ± 4.2 | 18.0 | 14.81 | 186.7 ± 9.2 | 7.4 |
| P2 | Protocatechuic aldehyde | 9.18 | 27.5 ± 2.0 | 33.4 | 6.12 | 72.1 ± 3.6 | 8.5 |
| P3 | Salvianolic acid D | 2.94 | >200 | 1.5 | 1.96 | 42.8 ± 2.2 | 4.6 |
| P4 | Salvianolic acid G | 2.74 | 116.2 ± 9.1 | 2.4 | 1.83 | >200 | 0.9 |
| P5 | Isolithospermic acid A | 2.68 | 51.0 ± 4.0 | 5.3 | 1.79 | 4.8 ± 0.4 | 37.1 |
| P6 | Salvianolic acid A | 2.43 | 23.7 ± 1.5 | 10.2 | 1.62 | 6.6 ± 0.5 | 24.5 |
| P7 | Isolithospermic acid B | 1.92 | 51.5 ± 3.6 | 3.7 | 1.28 | 20.0 ± 1.6 | 6.4 |
| P8 | Rosmarinic acid | 1.44 | 96.1 ± 7.2 | 1.5 | 0.96 | 36.9 ± 0.9 | 2.6 |
| P9 | Salvianolic acid B | 0.86 | 59.7 ± 3.9 | 1.4 | 0.57 | 8.8 ± 0.6 | 6.5 |
| P10 | Lithospermic acid | 0.65 | 146.2 ± 10.8 | 0.4 | 0.43 | 26.3 ± 1.2 | 1.6 |
| T1 | Tanshinone I | 0.44 | 9.2 ± 0.4 | 4.8 | 0.29 | 16.0 ± 1.2 | 1.8 |
| T2 | Dihydrotanshinone I | 0.39 | 2.8 ± 0.2 | 13.9 | 0.26 | 4.8 ± 0.3 | 5.4 |
| T3 | Tanshinone IIA | 0.30 | 17.5 ± 1.3 | 1.7 | 0.20 | 26.2 ± 2.1 | 0.8 |
| T4 | Cryptotanshinone | 0.24 | 8.0 ± 0.3 | 3.0 | 0.16 | 15.0 ± 0.7 | 1.1 |
ACI50 (%) = (Cn / C50,n) × 100. ACI50: activity contribution index at the median effect, is defined as activity contributions of single compounds in combination of PA and TN that inhibits 50%. Cn: concentration of Compoundn in combination of PA and TN that inhibits 50%. C50,n: also known as IC50, inhibitory concentration 50. The combination of PA and TN inhibited 50% of NO production at a corresponding concentration in 0.15 mg/ml CP, inhibited 50% of IL-6 production at a corresponding concentration in 0.10 mg/ml CP.