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. 2015 Jul 21;10(7):e0131799. doi: 10.1371/journal.pone.0131799

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© 2015 Tilaoui et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — PBMC were prepared from human normal donors by Ficoll-hypaque density centrifugation. Cells were incubated in 96-well microtiter plates in the presence of different essential oils (essential oil of leaves: EOL, essential oils of capitulum: EOC and essential oils of aerial parts: EOAP) at different concentrations (0, 4 μg/ml–50μg/ml). After 48 h incubation, viability was determined using MTT assay as described in materials and methods. Bar graph shows the mean percentage ± SD. *P < 0.05.