Skip to main content
. 2015 Jul 1;142(13):2364–2374. doi: 10.1242/dev.121913

Fig. 4.

Fig. 4.

Loss of CCN1 potentiates VEGF receptor activation. (A,B) Quantification by qPCR of endogenous levels of VEGF-A, VEGF-B and VEGF-C (A) and of VEGF-R1, VEGF-R2 and VEGF-R3 (B) mRNAs in CCN1+/+, iΔEC+/− and iΔEC−/− mouse retinas. Transcript levels in non-mutant CCN1+/+ were set to 100% to facilitate comparisons and analyses among the three groups of mice. *P<0.05 versus CCN1+/+ (n=5). (C,D) VEGF-R2 activation upon endothelial-specific CCN1 deletion in mice. Lysates from retinas were analyzed by immunoprecipitation with an anti-VEGF-R2 antibody followed by immunoblotting with either anti-pY1175 or anti-pY1214 antibody. VEGF-R2 protein band in the total protein input was visualized with anti-VEGF-R2 antibody. Densitometric quantification of the phosphorylated and non-phosphorylated forms of VEGF-R2 using ImageJ software is shown in D. **P<0.05 versus CCN1+/+ (n=3).