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. 2015 Jul 1;142(13):2291–2303. doi: 10.1242/dev.125237

Fig. 2.

Fig. 2.

Notch inhibition prevents floor plate but not motor neuron induction by notochord/ShhN. Schematic of the I-LNP dissection assay. (A) I-LNPs do not express cHairy2. (B) ShhN induces cHairy2 in I-LNP. This is inhibited by DAPT (C). (D-N) I-LNPs cultured alone (D,G,J) or in contact with a GFP+-notochord in DMSO (E,H,K,M) or DAPT (F,I,L,N). Serial sections analysed for Foxa2 (E,H) or Isl1 (F,I). (D,G,I,M) Isolated I-LNP does not express Foxa2 or Isl1. Notochord induction of Foxa2 (E) is inhibited by DAPT (F). Isl1 induction is not affected (H,I). (J) Isolated I-LNP does not express cNetrin1. Notochord induction of cNetrin1 (K) is inhibited by DAPT (L). (M,N) cT expression is unaffected by DAPT. (O-V) Sections of I-LNP explants. I-LNP explants cultured in 4 nM ShhN expressed both Foxa2 (P) and Isl1 (T). DAPT exposure prevented Foxa2 expression (Q) but maintained Isl1 (U). I-LNP explants cultured in 8 nM ShhN plus DAPT expressed both Foxa2 (R) and Isl1 (V). I-LNP, intermediate lateral neural plate tissue; cT, cBrachyury.