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. 2015 Jul 21;10(7):e0133274. doi: 10.1371/journal.pone.0133274

Fig 6. Kv4.2, Kv4.3 and KChIP2 mRNA expression level in ENDO and EPI tissue sample from left ventricular free wall of WT and Kv4.2-/- hearts.

Fig 6

Relative gene expression of voltage-gated K+ channels in ENDO and EPI tissues of WT (n = 6) and Kv4.2-/- (n = 6) hearts were measured by qtPCR and analyzed using the ∆∆Ct method with normalization to GAPDH, which did not change between samples. The qtPCR protocols involved 35 cycles for all reactions. All the primers were tested with by generating standard curves to measure probe efficiency and both meting curves and electrophoresis were utilized to confirm single PCR products. (A) This panel shows the estimated Kv4.2 mRNA expression levels detected in WT hearts. No specific Kv4.2 PCR product was detected in Kv4.2-/- hearts after 35 cycles (i.e. not detected, ND). Note the large EPI to ENDO gradients in the WT hearts, as expected. (B) Kv4.3 mRNA expression in both WT and Kv4.2-/- hearts showing no measureable gradient, although trends existing with more in the EPI than ENDO. (C) KChIP2 mRNA showing transmural heterogeneity (EPI>ENDO) in both the WT and Kv4.2-/- hearts.