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. 2010 Oct 28;3:29–56. doi: 10.4137/GEI.S4340

Table S2.

ESTs that tag for enzymatic functions putatively involved in the metabolism of unsaturated fatty acid of J. curcas.

Pathway EST Reads/contig KEGG homolog Gene definition EC Map Sz1 Homol2 Id%3
Biosynthesis of unsaturated fatty acids (01040)
Contig332 FM890615, FM896803, FM892730 ath:AT3G06860 Multifunctional protein MFP2 1.1.1.211 1070, 71, 1040, 62 725 258 84
*Contig376 FM891097, FM89225, FM891175, FM893271, FM892235, FM893697 rcu:RCOM_1431360 Short chain dehydrogenase 1.1.1.100 1040, 61 293 181 88
*Contig533 FM892613, FM894348, FM895006, FM890906, FM892529 rcu:RCOM_0472100 Short chain alcohol dehydrogenase 1.1.1.100 1040, 61 280 277 78
FM889551 rcu:RCOM_1636080 2,4-dienoyl-CoA reductase 1.1.1.100 1040, 61 285 109 88
FM891538 rcu:RCOM_1431360 Short chain dehydrogenase 1.1.1.100 1040, 61 293 56 80
FM892914 rcu:RCOM_1020840 3-ketoacyl-CoA thiolase B 2.3.1.16 1070, 71, 592, 1040, 62 460 88 90
FM894979 pop:POPTR_832274 Acyl-CoA oxidase 1.3.3.6 1070, 71, 592, 1040 689 196 92
GO247153 olu:OSTLU_12103 3-oxoacyl- [acyl-carrier protein reductase 1.1.1.100 1040, 61 273 44 77
GT228627 rcu:RCOM_1081890 Ketoacyl-ACP Reductase 1.1.1.100 1040, 61 328 188 75
GT229218 rcu:RCOM_0696050 Enoyl-CoA hydratase, mitoc. precursor 4.2.1.17 1070, 71, 592, 1040, 62 389 195 78
Linoleic acid metabolism (00591)
FM888497 rcu:RCOM_0597840 Desacetoxyvindoline 4-hydroxylase 1.14.11.20 1063, 901 361 166 69
FM889983 rcu:RCOM_0603110 Polyneuridine- aldehyde esterase precursor 3.1.1.78 901 260 144 60
GO246771 rcu:RCOM_0602630 Polyneuridine- aldehyde esterase precursor 3.1.1.78 901 263 152 67
Alpha-Linolenic acid metabolism (00592)
Contig332 FM890615, FM896803, FM892730 ath:AT3G06860 Multifunctional protein MFP2 4.2.1.17 1070, 71, 592, 1040, 62 725 258 84
*Contig369 FM891024, FM895943, FM893216, FM892025 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 63 77
*Contig682 FM894273, FM896105, FM89466 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 176 80
*Contig859 FM895802, FM894994, FM889185 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 153 80
*Contig938 FM896585, FM893098, FM889131, FM889465 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 98 84
FM887427 pop:POPTR_550801 Lysine-specific histone demethylase 1 1.-.-.- 592, 950 795 47 68
FM887948 ath:AT3G59840 Hypothetical protein 1.-.-.- 592, 950 97 75 61
FM892914 rcu:RCOM_1020840 3-ketoacyl-CoA thiolase B 2.3.1.16 1070, 71, 592, 1040, 62 460 88 90
FM894979 pop:POPTR_832274 Acyl-CoA oxidase 1.3.3.6 1070, 71, 592, 1040 689 196 92
FM895499 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 135 77
FM896436 rcu:RCOM_1016730 Cytochrome P450 4.2.1.92 1070, 592 496 140 80
FM896469 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 87 65
FM896520 xtr:595069 Fa2h; fatty acid 2-hydroxylase 1.-.-.- 592, 950 371 139 52
GR209288 rcu:RCOM_0721980 Amine oxidase, lysine-specific histone demethylase 1 1.-.-.- 592, 950 961 63 80
GT228436 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 183 68
GT229029 rcu:RCOM_1593180 Cytochrome P450 4.2.1.92 1070, 592 518 46 89
GT229218 rcu:RCOM_0696050 Enoyl-CoA hydratase, mitoc. precursor 4.2.1.17 1070, 71, 592, 1040, 62 389 195 78
Arachidonic acid metabolism (00590)
*Contig326 FM890550, FM893788, GT229087, FM888006, FM895876, FM894387, FM889334, FM895717, FM890427 tgo:TGME49_067680 Microneme protein 1.14.99.1 590 2182 48 54
*Contig369 FM891024, FM895943, FM893216, FM892025 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 63 77
*Contig429 FM891645, FM889741, GO246578, FM889668 rcu:RCOM_0902850 Short-chain dehydrogenase 1.1.1.184 590 253 156 70
*Contig682 FM894273, FM896105, FM89466 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 176 80
*Contig859 FM895802, FM894994, FM889185 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 153 80
*Contig938 FM896585, FM893098, FM889131, FM889465 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 98 84
*Contig1156 GO246787, FM895785, FM896209, FM890569, FM894435, GH295599, FM895852, FM893178, GH295993, GH296534, FM894252, FM888871, FM890857, FM894719 pop:POPTR_749200 Glutathione peroxidase 1.11.1.9 590 251 238 76
FM894424 rcu:RCOM_1437260 Cytochrome P450 1.14.14.1 1063 632 180 86
FM895499 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 135 77
FM896469 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 87 65
GT228436 cre:CHLREDRAFT_185967 FTT1; 14-3-3 protein 3.1.1.4 564, 590, 592, 565, 591 259 183 68
GT229226 rcu:RCOM_1503180 Carbonyl reductase 1.1.1.189 590 315 110 80

Notes:

1

Sz is for the size of the KEGG homologous protein in amino acids;

2

Homol is for the size of the homologous region in amino acids;

3

Id% is for the pourcentage of identity of amino acid homologous pairs;

*

A contig with an asterisk in front means that its reads show nucleotide polymorphism.