Table S5.

ESTs that tag for enzymatic functions putatively involved in alkaloid biosynthesis in J. curcas.

Pathway	EST	Reads/contig	KEGG homolog	Definition	EC	Map	Sz1	Homol2	Id%3
Indole alkaloid biosynthesis (00901)
	FM888497		rcu:RCOM_0597840	Desacetoxyvindoline 4-hydroxylase	1.14.11.20	1063, 901	361	166	69
	FM889983		rcu:RCOM_0603110	Polyneuridine-aldehyde esterase precursor	3.1.1.78	901	260	144	60
	GO246771		rcu:RCOM_0602630	Polyneuridine-aldehyde esterase precursor	3.1.1.78	901	263	152	67
Isoquinoline alkaloid biosynthesis (00950)
	*Contig794	FM895278, FM895972, FM895925, FM895522, FM895639	rcu:RCOM_1052360	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	404	276	90
	Contig872	FM895919, FM896552	rcu:RCOM_0770830	Reticuline oxidase precursor	1.21.3.3	1063, 950	524	251	72
	*Contig930	FM896500, FM896046	rcu:RCOM_1173140	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	419	131	83
	*Contig1301	GR209293, GR209295	rcu:RCOM_0536450	Amine oxidase	1.4.3.4	950, 982	491	150	88
	FM887321		rcu:RCOM_1682160	Cytochrome P450	1.14.13.71	950	501	83	57
	FM887370		rcu:RCOM_0651460	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	415	110	75
	FM887427		pop:POPTR_550801	Lysine-specific histone demethylase 1	1.-.-.-	592, 950	795	47	68
	FM887948		ath:AT3G59840	Hypothetical protein	1.-.-.-	592, 950	97	75	61
	FM888061		rcu:RCOM_1173140	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	419	83	79
	FM888095		vvi:100242364	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	462	220	91
	FM890598		pop:POPTR_896773	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	406	84	86
	FM890776		rcu:RCOM_0922300	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	464	46	97
	FM893208		reh:H16_A1025	Rossmann fold nucleotide-binding protein/lysine decarboxylase family protein	4.1.1.25	1063, 950	197	94	52
	FM894218		rcu:RCOM_1574460	S-N-methylcoclaurine 3′-hydroxylase isozyme	1.14.13.71	950	318	162	66
	FM896388		rcu:RCOM_0984440	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	484	104	80
	FM896520		xtr:595069	Fa2h; fatty acid 2-hydroxylase	1.-.-.-	592, 950	371	139	52
	FM896620		rcu:RCOM_1335460	Reticuline oxidase precursor	1.21.3.3	1063, 950	539	165	72
	GO246518		rcu:RCOM_0802970	Aspartate aminotransferase	2.6.1.1	1070, 1064, 710, 950	440	121	85
	GR209288		rcu:RCOM_0721980	Amine oxidase, lysine-specific histone Demethylase 1	1.-.-.-	592, 950	961	63	80
	GT228598		rcu:RCOM_1081680	3′-N-debenzoyl-2′-deoxytaxol N-benzoyltransferase	2.3.1.150	1063, 950	437	45	75
	GT229047		rcu:RCOM_1335910	Reticuline oxidase precursor	1.21.3.3	1063, 950	548	186	73
Ubiquinone and other terpenoid-quinone biosynthesis (00130)
	*Contig270	FM889998, FM890294	rcu:RCOM_1328840	AMP dependent ligase	6.2.1.26	130	556	108	80
	*Contig930	FM896500, FM896046	rcu:RCOM_1173140	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	419	131	83
	Contig1079	GH295872, GH296266, GH296452	rcu:RCOM_0999850	AMP dependent ligase	6.2.1.26	130	564	81	87
	FM887370		rcu:RCOM_0651460	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	415	110	75
	FM888061		rcu:RCOM_1173140	Tyrosine aminotransferase	2.6.1.5	130, 950, 1063, 1064	419	83	79
	FM889157		rcu:RCOM_1192240	4-hydroxyphenylpyruvate dioxygenase	1.13.11.27	130	441	171	53
	FM893861		rcu:RCOM_1328840	AMP dependent ligase	6.2.1.26	130	556	158	81
	FM896109		ath:AT3G48360	BT2; BTB and TAZ domain protein 2 (protein binding/transcription factor/transcription regulator)	1.14.-.-	130, 1070, 1062, 100, 904, 905	364	87	50
	GO246531		rcu:RCOM_1495910	AMP dependent CoA ligase	6.2.1.26	130	521	80	56

Notes:

¹Sz is for the size of the KEGG homologous protein in amino acids;

²Homol is for the size of the homologous region in amino acids;

³Id% is for the pourcentage of identity of amino acid homologous pairs;

^*A contig with an asterisk in front means that its reads show nucleotide polymorphism.