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. 2010 Oct 28;3:29–56. doi: 10.4137/GEI.S4340

Table S4.

ESTs that tag for enzymatic functions putatively involved in terpene biosynthesis in J. curcas.

Pathway EST Reads/contig KEGG homolog Definition EC Map Sz1 Homol2 Id%3
Terpenoid backbone biosynthesis (00900)
*Contig1203 GO247043, GH295921, FM896506, GH296298, GH295905, GH296485, GH296314, GH296501 swo:Swol_1344 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/S1 RNA-binding domain protein 1.17.1.2 1070, 1062, 1066, 900 687 54 51
FM888319 pop: POPTR_418371 1-deoxy-D-xylulose-5-phosphate synthase 2.2.1.7 1070, 1062, 1066, 900 657 64 93
FM889171 pop: POPTR_646543 Hydroxymethylglutaryl-CoA synthase 2.3.3.10 1070, 1062, 1066, 900 464 112 92
FM894459 rcu:RCOM_1431350 Mevalonate kinase 2.7.1.36 1070, 1062, 1066, 900 386 60 83
GO246785 rcu:RCOM_0679170 1-deoxyxylulose-5-phosphate synthase 2.2.1.7 1070, 1062, 1066, 900 720 81 90
GT229261 rcu: RCOM_0747390 Geranylgeranyl pyrophosphate synthase 2.5.1.30 900 417 102 83
Monoterpenoid biosynthesis (00902)
FM887640 rcu:RCOM_1544790 R-limonene synthase 4.2.3.20 1062, 902 596 45 80
FM888507 rcu: RCOM_1732250 R-limonene synthase 4.2.3.20 1062, 902 250 54 44
FM888632 rcu: RCOM_1593840 Cytochrome P450 1.3.3.9 1062, 1066, 902 613 64 65
FM889984 rcu: RCOM_1544790 R-limonene synthase 4.2.3.20 1062, 902 596 72 75
Diterpenoid biosynthesis (00904)
FM887587 rcu: RCOM_1013800 3′-N-debenzoyl-2′-deoxytaxol N-benzoyltransferase 2.3.1.167 1062, 904 446 86 89
FM889243 rcu: RCOM_1607300 Cytochrome P450 1.14.13.79 1070, 1062, 904 298 44 84
FM891970 rcu: RCOM_1574750 Casbene synthase, chloroplast precursor 4.2.3.8 1062, 904 555 138 69
FM896109 ath:AT3G48360 BT2; BT2 (BTB and TAZ domain protein 2; protein binding/transcription factor/transcription regulator) 1.14.-.- 1070, 1062, 100, 904, 130, 905 364 87 50
Sesquiterpenoid biosynthesis (00909)
FM889102 rcu:RCOM_1045160 Delta-cadinene synthase isozyme A 4.2.3.13 1062, 909 547 112 59

Notes:

1

Sz is for the size of the KEGG homologous protein in amino acids.

2

Homol is for the size of the homologous region in amino acids.

3

Id% is for the pourcentage of identity of amino acid homologous pairs;

*

A contig with an asterisk in front means that its reads show nucleotide polymorphism.