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. 2015 Jul 21;10(7):e0132948. doi: 10.1371/journal.pone.0132948

Fig 5. The observation that ECM elevates response applies to native systems.

Fig 5

(A) Average P3-yfp and PrpsD-yfp as a function of normalized PtapA activity (mean ± SEM, total n = 646 cells). WT Receiver cells containing PtapA-cfp were grown in liquid MSgg culture with 1μM AIP. The expression from the reporters PtapA-cfp and P3-yfp were measured for individual cells from microscopic snapshots. Relationship between PtapA-cfp and PrpsD-yfp were measured accordingly using the strain containing PtapA-cfp and PrpsD-yfp. (B) Response of WT and ΔepsH to conditioned media, which contains native quorum sensing signals that activate Psrf. Response are measured by Psrf-lacZ (mean ± SEM, n = 6, p<0.01 for native QS signals + group). (C) Model: ECM production can exclusively benefit producer cells by enhancing their response to QS, thereby compensating for the cost of ECM production.