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. 2015 Jul 8;6:7624. doi: 10.1038/ncomms8624

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RgNanH was incubated with (a) 4MU-Neu5Ac or (b) 3′SL at 37 °C, pH 6.5, overnight and the reaction mixture analysed by H1 NMR. Control reactions without enzyme (‘—', lower trace) were carried out in parallel with the reaction containing enzyme (‘+', upper trace). ‘A' corresponds to 2,7-anhydro-Neu5Ac, see c for chemical structure and NMR shifts.