Figure 2. Alleviation of autoimmune disease in cKO mice.

(a) Schematic representation of the miR-31 locus and targeting strategy. Cre-mediated recombination of loxP sites in mice. Primers (P1 and P2) spanning the loxP sites were designed for genotyping floxed allele (1,195 bp) and deleted allele (474 bp). (b) PCR products of splenocytes and sorted CD4⁺ T cells derived from either miR-31^fl/fl control or miR-31^fl/flCD4^Cre (cKO) mice. (c) qPCR analysis to confirm the deletion of miR-31 in CD4⁺ T cells derived from cKO mice. (d,e) Clinical scores and weight loss (mean±s.e.m.) of miR-31^fl/fl or cKO mice after the induction of EAE were assessed every day (n=7 per group). ***P<0.001, two-tailed Student's t-test for c, one-way analysis of variance for d and e. Data are representative of two (c) or three (b,d and e) independent experiments (mean±s.e.m.).