Fig. 4.

PDK3 overexpression causes metabolic dysfunction in oocytes. (A) Histogram showing the total ATP content in control and PDK-overexpressing oocytes (n=50 for each group). (B) Effects of PDK overexpression on intracellular ROS levels, as estimated by CM-H2DCFDA fluorescence intensity. (C) Representative images of CM-H2DCFDA fluorescence in oocytes analyzed in B. For A and B, at least 50 oocytes were examined for each group, data are expressed as the mean±s.d. from three independent experiments, *P<0.05 versus controls. Scale bar: 50 µm.