Fig. 8.

Diagram illustrating how experimental perturbations of PDK paralog activity affect site-specific phosphorylation for PDHE1α and oocyte functions. Overexpression of PDK3 (upward arrow), but not knockdown, results in increased pSer293-PDHE1α, which leads to decreased PDH activity resulting in decreased total ATP, increased ROS and spindle assembly defects and chromosome misalignment in oocytes. Knockdown of PDK1 or PDK2 (downward arrow), but not overexpression, results in decreased pSer232-PDHE1α, which leads to spindle assembly defects and chromosome misalignment in metaphase oocytes. See text for details.