Fig. 2.

Reporter dynamics after exposure of 10T1/2 cells to different growth factors. (A) Time course of the relative nuclear intensity of the FoxO1–clover reporter in cells incubated in SFM and then exposed to SFM, BMP-2 (15 nM), R3-IGF-I (1 nM), 10% FBS or PDGF-BB (206 pM) for 60 min. Population means are presented (n=50 cells per incubation). The nuclear intensity of the reporter in each cell was normalized to its value at the start of imaging during incubation in SFM. (B) Expression of phosphorylated Akt (pAkt, at Thr308), total Akt, phosphorylated Smad5 (pSmad5), total Smad (Smad1,5,8) and α-tubulin by immunoblotting using whole-cell protein lysates from the same population analyzed in A after exposure to SFM or the indicated growth factors for 60 min. The position of the 50 kDa molecular mass marker is indicated to the right of each immunoblot.