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. 2015 Jul 15;128(14):2509–2519. doi: 10.1242/jcs.168773

Fig. 7.

Fig. 7.

The FoxO1–clover reporter actively shuttles between the nucleus and cytoplasm. (A) Time course of the relative nuclear intensity of the FoxO1–clover reporter in 10T1/2 cells incubated with SFM or R3-IGF-I (250 pM) as indicated for 60 min, followed by addition of leptomycin B (100 nM, Lepto) alone or with PI-103 (500 nM) for 180 min. Population means are presented (n=50 cells per incubation). The arrow indicates the time of addition of Lepto or PI-103. (B) Time course of relative nuclear intensity of the FoxO1–clover reporter in 10T1/2 cells incubated with SFM or R3-IGF-I (250 pM) at time 0 followed by a wash and addition of SFM or PI-103 at 30 min. Cells were imaged every 2 min in both A and B.