Fig. 7.

The stem cell-dependent Smed-wnt1-expressing cells also express Smed-junl-1. Smed-junl-1 does not colocalise with Smed-wnt1 in the wound-induced phase of wnt1 expression (24 hR) (nc=43/43 wnt1⁺ cells in four animals), but becomes colocalised from 2 dR onwards (2 dR co=17/20 wnt1⁺ cells in four animals; 3 dR co=87/87 in 16 animals over four experiments; 4 dR co=21/21 in four animals). Arrowheads indicate cells with co-expression of wnt1 and junl-1, except at 24 hR where the arrowhead highlights a single junl-1-expressing cell. Shown are posterior blastemas from regenerating head pieces. One plane, as obtained by confocal microscopy, is shown. Counts indicate the number of cells showing colocalised junl-1/wnt1 expression (co) or no colocalisation (nc). Scale bars: 50 μm.