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. 2015 Jul 15;142(14):2521–2532. doi: 10.1242/dev.120220

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© 2015. Published by The Company of Biologists Ltd

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Fig. 6. — Co-expression of Shox2 with cTnT, Hcn4 and Nkx2-5 in differentiated pacemaker-like cells in EBs. (A) Shox2 expression, as revealed by DsRed (arrowheads), in an EB differentiated from stem cells bearing the Shox2^HA allele. (B,C) Immunocytochemistry shows colocalization of Shox2 with cTnT, Nkx2-5 and Hcn4. (B) White arrowheads point to Shox2⁺/cTnT⁺/Nkx2.5⁺ cells, and open arrowheads point to Shox2⁺/cTnT⁺ cells. (C) White arrowhead points to a Shox2⁺/Nkx2.5⁺/Hcn4⁺ cell, and open arrowhead points to a Shox2⁺/Hcn4⁺ cell. (D) Percentage of cells that co-express Shox2 and other molecular markers. (E) qPCR reveals elevated Hcn4 expression in differentiated EBs with Nkx2-5 silencing by siRNA. **P<0.01.