Fig. 7.

Genome-wide co-occupancy by Shox2 and Nkx2-5. (A) GO analysis of phenotypes of annotated genes associated with Shox2 binding peaks. (B,C) Genome-wide co-occurrence of Shox2, Nkx2-5 and Tbx5 binding sites. (D) Plot of Shox2, Nkx2-5 and Tbx5 co-bound peaks around TSSs. (E) Co-occurrence of Shox2, Nkx2-5 and Tbx5 binding sites on representative genes (read numbers above 0.15/million reads are shown). (F) Co-immunoprecipitation shows physical interaction between Shox2 and Nkx2-5. (G) Direct co-regulation of target genes by Shox2, Nkx2-5 and Tbx5 underlies the Shox2–Nkx2-5 antagonistic mechanism.