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tbx16-EnR expression represses sox2 and activates ntl. In situ hybridization shows changes to gene expression with HS:tbx16-EnR (B,D,F,H,J) or in control (A,C,E,G,I) 4 h after heat shock. The red boxes (B,D,F,H) highlight the reduction of tbx6l, pcdh8, her1 and sox2 in the tailbud and the arrow (J) points to an expansion of ntl.
