Fig. 8.

sem-2 affects the development of RME motor neurons. (A) The expression of terminal differentiation markers of RME identity is affected in sem-2 mutants. Reporter genes were crossed into sem-2(ok2422) null mutants. The fraction of animals that show the indicated phenotype is indicated in the bar charts. Animals were scored at larval stages; n>30. (B) The fate of other neurons that have a sem-2-expressing progenitor is unaffected in sem-2(ok2422) mutants. Animals were scored at larval stages. (C) RMEL/R neurons are not generated in sem-2 mutants. Wild-type and sem-2(ok2422) mutant animals were crossed with the pan-neuronal marker rab-3 (red) and the glutamatergic marker eat-4 (green). eat-4 expression was used as a landmark to assess rab-3 expression in RME neurons.