Table 3. High risk Human Papillomavirus types per hot-spot mutation and per histological subtype.

Gene	Mutation	SCC	AC	ASC
PIK3CA, n = 61 (20%)	p.E545K, n = 40	negative (2)	16	16 (4)
		16 (15)	18 (2)	18
		18 (4)		31
		45		33
		51		16+68
		52
		73
		16+31
		16+68+73
		18+45
		51+52
	p.E542K, n = 17	16 (8)	16 (2)	16 (3)
		18		31
		31
		52
	p.H1047R, n = 4	31	18	51
		33
	p.R88Q, n = 1	negative
KRAS, n = 20 (7%)	p.G12D, n = 10	16	negative (2)	18 (2)
		52	16 (3)
			18
	p.G13S, n = 4		16 (3)
			18
	p.G12V, n = 3	negative	18
		16
	p.G12C, n = 1	16
	p.G12A, n = 1		16
	p.G12S, n = 1		18
PTEN, n = 12 (4%)	p.R130G, n = 3	negative	negative (2)
	p.Q214*, n = 2	negative		16
	p.R234W, n = 2	negative		16
	p.R130*, n = 1	16
	p.R130fs*4, n = 1		18
	p.R173H, n = 1		16
	p.K267fs*31, n = 1		16+18
	p.K267fs*9, n = 1			16
PPP2R1A, n = 9 (3%)	p.R258H, n = 6	16	16	16
		16+52		18
		31
	p.P179R, n = 3	16	18
		33
	p.R183W, n = 1	16
CTNNB1, n = 8 (3%)	p.G34R, n = 3	16		16
		45
	p.D32N, n = 1	16
	p.S37F, n = 1	16
	p.S45F, n = 1	negative
	p.T41A, n = 1	33
	p.T41I, n = 1	16
CDKN2A, n = 4 (1%)	p.R58*, n = 2	negative	45
	p.P114L, n = 1	16
	p.W110*, n = 1		16
FBXW7, n = 3 (1%)	p.R465H, n = 2	negative
		16
	p.R465C, n = 1		18
FGFR3, n = 2 (1%)	p.S249C, n = 2	negative		16
HRAS, n = 1 (<1%)	p.G12D, n = 1			16+33
NRAS, n = 1 (<1%)	p.Q61R, n = 1	16

SCC, squamous cell carcinoma; AC, adenocarcinoma; ASC, adenosquamous carcinoma. When more than one tumor with the same mutation and same HPV type was detected, the number is given between brackets. Seven tumors were double positive, one tumor was triple positive, shown with"+".