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. 2015 Jul 22;34(1):69. doi: 10.1186/s13046-015-0186-x

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© Broecker-Preuss et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Increased activity of caspase 3 and 7 (a), increase in the concentration of cleaved caspase 3 fragments as a sign of progressing caspase activity (b) and increased LDH-release into the cell culture medium (c) after treatment with 0.1 μM GX15-070 for 2, 6, 16 and 24 h in different thyroid carcinoma cell lines. Values were determined by Apo-One assay, an ELISA specific for caspase 3 fragments and CytoTox-assay and are depicted as percent of vehicle-treated control. Data represent mean values of eight-fold determinations ± standard deviation; * indicates significant increase (p < 0.05, Student’s t-test)