Table 2.
Distribution of cell cycle phases in vehicle-treated thyroid carcinoma cells and cells treated for 24 h with 0.1 μM GX15-070
| Cell line | Stimulation | % subG1 | % G1 | % G2/M | % S |
|---|---|---|---|---|---|
| FTC236 (FTC) | unstimulated | 0.3 ± 0.04 | 36.7 ± 2.4 | 17.0 ± 1.1 | 46.3 ± 3.1 |
| 24 h GX15-070 | 9.9 ± 2.4 * | 81.9 ± 5.3 * | 12.2 ± 1.7 * | 6.0 ± 0.9 * | |
| ML1 (FTC) | unstimulated | 1.2 ± 0.3 | 39.4 ± 1.7 | 28.5 ± 1.5 | 32.1 ± 1.8 |
| 24 h GX15-070 | 34.8 ± 3.5 * | 82.2 ± 5.4 * | 14.3 ± 0.9 * | 3.5 ± 0.4 * | |
| BHT101 (PTC) | unstimulated | 3.7 ± 0.6 | 52.5 ± 3.9 | 16.3 ± 1.1 | 31.3 ± 2.2 |
| 24 h GX15-070 | 27.9 ± 3.0 * | 76.1 ± 5.4 * | 15.4 ± 1.4 | 8.6 ± 1.0 * | |
| SW1736 (ATC) | unstimulated | 2.4 ± 0.2 | 35.9 ± 1.7 | 24.3 ± 2.0 | 39.8 ± 2.5 |
| 24 h GX15-070 | 9.3 ± 1.3 * | 77.5 ± 5.5 * | 11.8 ± 0.9 * | 10.8 ± 1.1 * | |
| C643 (ATC) | unstimulated | 0.7 ± 0.1 | 54.5 ± 4.2 | 13.9 ± 0.9 | 38.6 ± 2.7 |
| 24 h GX15-070 | 21.7 ± 2.7 * | 75.6 ± 5.8 * | 10.4 ± 1.2 * | 14.0 ± 0.9 * | |
| HTh7 (ATC) | unstimulated | 1.1±0.2 | 55.5 ± 3.9 | 11.3 ± 0.9 | 33.2 ± 1.8 |
| 24 h GX15-070 | 4.1 ± 0.3 * | 80.0 ± 5.7 * | 3.9 ± 0.2 * | 16.1 ± 1.0 * |
Values for G1-, G2/M-, and S-phases are determined for the living cells that were not included in the sub-G1-peak. * indicates significant changes (p < 0.05, Student’s t-test)