Skip to main content
. 2015 Jul 13;6:7721. doi: 10.1038/ncomms8721

Figure 7. PDGF regulates directional cell migration via the P-switch.

Figure 7

(a) A western blot showing the expression of PDGFR-β in MDA-MB-231, but not in MCF10A. (b) Acute PDGF treatment (30 min) following serum starvation led to phosphorylation of Erk1/2 and activation of RhoA and Rac1. (c) PDGF regulates the dynamic interactions of DLC1, TNS3, PTEN and PI3K. (d) A western blot showing the knockdown of DLC1 in MDA-MB-231 cells. (e) A ‘defective' P-switch in DLC1-depleted MDA-MB-231: PI3K–p85 was found to bind both PTEN and TNS3 in the absence of DLC1 following PDGF stimulation. (f) Phase-contrast images of MCF10A and MDA-MB-231 cells transfected with control or DLC1-specific siRNA at 0 and 16 h after wounding by scratch. Cells were cultured in serum-free medium with PDGF. (g) Confocal images of MDA-MB-231 cells with or without depletion of DLC1 showing the distinct patterns of actin network at the edge of the scratch wound. Scale bar, 10 μm (white), 100 μm (red). Data shown are representative of three independent experiments.