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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Int J Dev Biol. 2014;58(9):693–699. doi: 10.1387/ijdb.140288jp

Fig. 4. Expression of sox9b:EGFP in the heart.

Fig. 4

(A–D) Confocal images of sox9b:EGFP expression in embryonic, larval, and adult zebrafish hearts. Cardiomyocytes are marked with activated leukocyte cell adhesion molecule (ALCAM; red in A–C) and nuclei are stained with DAPI (blue); (A) Lateral view of a 24 hpf embryo. The developing heart is outlined and indicated by the arrow. (B,C) Ventral views of a 72 and 96 hpf hearts. Expression of sox9b:EGFP is detected in cardiomyocytes, the bulbus arteriosus (arrowheads in B and C), and in epicardial cells (arrows in C). (D) Ventral view of an adult heart. Expression of sox9b:EGFP in cardiomyocytes continues into adulthood. V is ventricle; At, atrium; BA, bulbus arteriosus. Anterior is to the left in all panels. Scale bars, 50 microns.