Table 1.
Detailed inventory of the Vitis MAPK cascade proteins.
| Subfamily name | 12X Vitis vinifera ID | NCBI GenBank ID | Chr | Str | Genomic location | Gene length in bp | CDS length in bp | Length of protein in AA | Number of Exon | Number of Intron | pI | mW (kDa) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VvMPKs | ||||||||||||
| VvMPK1 | GSVIVT01000784001 | CBI31754.3 | 12 | + | 124452–133238 | 8787 | 1518 | 505 | 10 | 9 | 9.34 | 57.43 |
| VvMPK2 | GSVIVT01005924001 | CBI35594.3 | 7 | + | 886169–898284 | 12116 | 1341 | 446 | 16 | 15 | 5.40 | 51.22 |
| VvMPK3 | GSVIVT01008408001 | CBI15552.3 | 17 | + | 2368190–2377747 | 9558 | 1806 | 601 | 11 | 10 | 6.89 | 67.98 |
| VvMPK4 | GSVIVT01009766001 | CBI19748.3 | 18 | + | 11125765–11129338 | 3574 | 588 | 195 | 4 | 3 | 5.44 | 22.50 |
| VvMPK5 | GSVIVT01011749001 | CBI26902.3 | 1 | − | 4565334–4574753 | 9420 | 1842 | 613 | 11 | 10 | 8.68 | 70.46 |
| VvMPK6 | GSVIVT01014081001 | CBI20098.3 | 19 | + | 224299–234190 | 9892 | 1797 | 599 | 10 | 9 | 9.21 | 67.79 |
| VvMPK7 | GSVIVT01017873001 | CBI26170.3 | 5 | − | 4205509–4215917 | 10409 | 1692 | 563 | 10 | 9 | 8.59 | 64.03 |
| VvMPK8 | GSVIVT01018883001 | CBI17457.3 | 4 | + | 18974001–19005635 | 31635 | 2310 | 769 | 10 | 9 | 5.51 | 87.48 |
| VvMPK9 | GSVIVT01019406001 | CBI34380.3 | 2 | − | 380310–386888 | 6579 | 1128 | 375 | 6 | 5 | 5.86 | 42.80 |
| VvMPK10 | GSVIVT01022771001 | CBI37450.3 | 2 | + | 16326975–16335400 | 8426 | 1359 | 452 | 16 | 15 | 9.62 | 51.58 |
| VvMPK11 | GSVIVT01025091001 | CBI16237.3 | 6 | + | 4580755–4584961 | 4207 | 1116 | 371 | 6 | 5 | 4.94 | 42.53 |
| VvMPK12 | GSVIVT01025105001 | CBI16244.3 | 6 | − | 4432854–4436338 | 3485 | 990 | 329 | 6 | 5 | 5.52 | 38.17 |
| VvMPK13 | GSVIVT01026984001 | CBI40425.3 | 15 | − | 18821560–18826926 | 5367 | 1128 | 375 | 6 | 5 | 6.43 | 43.27 |
| VvMPK14 | GSVIVT01038192001 | CBI24707.3 | 5 | + | 24220238–24241107 | 20870 | 993 | 330 | 6 | 5 | 5.64 | 38.37 |
| VvMAPKKs | ||||||||||||
| VvMKK1 | GSVIVT01008476001 | CBI15608.3 | 17 | + | 1537423–1538551 | 1129 | 675 | 224 | 3 | 2 | 6.38 | 24.66 |
| VvMKK2 | GSVIVT01015155001 | CBI27870.3 | 11 | + | 1417439–1424337 | 6899 | 1065 | 355 | 8 | 7 | 6.00 | 39.28 |
| VvMKK3 | GSVIVT01015283001 | CBI27984.3 | 11 | + | 2377698–2381398 | 3701 | 1065 | 355 | 8 | 7 | 6.02 | 39.98 |
| VvMKK4 | GSVIVT01016115001 | CBI25274.3 | 9 | + | 19257788–19265261 | 7474 | 1188 | 396 | 5 | 4 | 10.15 | 43.78 |
| VvMKK5 | GSVIVT01032414001 | CBI34873.3 | 14 | − | 27139003–27145873 | 6871 | 1557 | 519 | 9 | 8 | 5.56 | 57.61 |
| VvMAPKKKs | ||||||||||||
| VviMAPKKK1 | GSVIVT01000047001 | CBI36768.3 | 14 | + | 3063647–3072319 | 8673 | 1992 | 664 | 17 | 16 | 5.36 | 72.89 |
| VviMAPKKK2 | GSVIVT01000256001 | CBI27711.3 | 7 | + | 20596048–20597073 | 1026 | 921 | 307 | 2 | 1 | 8.97 | 33.93 |
| VviMAPKKK3 | GSVIVT01001193001 | CBI28728.3 | 7 | + | 944892–950225 | 5334 | 1215 | 405 | 6 | 5 | 7.03 | 44.92 |
| VviMAPKKK4 | GSVIVT01001690001 | CBI35506.3 | 18 | − | 14296312–14329573 | 33262 | 1653 | 551 | 16 | 15 | 5.07 | 61.80 |
| VviMAPKKK5 | GSVIVT01002332001 | CBI35719.3 | Un | + | 34161697–34167622 | 5926 | 696 | 232 | 7 | 6 | 9.43 | 26.14 |
| VviMAPKKK6 | GSVIVT01004254001 | CBI18826.3 | Un | + | 37734319–37739476 | 5158 | 1158 | 386 | 10 | 9 | 9.51 | 43.07 |
| VviMAPKKK7 | GSVIVT01007446001 | CBI25853.3 | Un | + | 31988209–31995727 | 7519 | 2124 | 708 | 11 | 10 | 9.73 | 77.57 |
| VviMAPKKK8 | GSVIVT01007637001 | CBI14941.3 | 17 | − | 10966272–10980533 | 14262 | 1464 | 488 | 9 | 8 | 5.47 | 54.86 |
| VviMAPKKK9 | GSVIVT01007646001 | CBI14949.3 | 17 | + | 10874999–10877438 | 2440 | 1059 | 353 | 6 | 5 | 8.13 | 40.08 |
| VviMAPKKK10 | GSVIVT01007762001 | CBI15038.3 | 17 | + | 9308908–9314007 | 5100 | 909 | 303 | 3 | 2 | 7.97 | 34.37 |
| VviMAPKKK11 | GSVIVT01007775001 | CBI15048.3 | 17 | − | 9166428–9172256 | 5829 | 1050 | 350 | 6 | 5 | 7.02 | 38.56 |
| VviMAPKKK12 | GSVIVT01008413001 | CBI15555.3 | 17 | − | 2321687–2342403 | 20717 | 2697 | 899 | 16 | 15 | 5.24 | 99.09 |
| VviMAPKKK13 | GSVIVT01008728001 | CBI18907.3 | 18 | − | 1477098–1491666 | 14569 | 1569 | 523 | 16 | 15 | 6.67 | 59.46 |
| VviMAPKKK14 | GSVIVT01008938001 | CBI19081.3 | 18 | − | 3594893–3606331 | 11439 | 822 | 274 | 8 | 7 | 6.45 | 30.15 |
| VviMAPKKK15 | GSVIVT01009192001 | CBI19282.3 | 18 | − | 5939861–5949524 | 9664 | 2718 | 906 | 15 | 14 | 8.41 | 101.48 |
| VviMAPKKK16 | GSVIVT01009575001 | CBI19581.3 | 18 | + | 9549009–9561275 | 12267 | 1101 | 367 | 10 | 9 | 8.39 | 40.91 |
| VviMAPKKK17 | GSVIVT01012031001 | CBI27127.3 | 1 | − | 2006896–2039042 | 32147 | 4191 | 1397 | 25 | 24 | 5.73 | 154.60 |
| VviMAPKKK18 | GSVIVT01012116001 | CBI27196.3 | 1 | + | 1303636–1315494 | 11859 | 3717 | 1239 | 11 | 10 | 5.32 | 136.73 |
| VviMAPKKK19 | GSVIVT01012632001 | CBI23172.3 | 10 | + | 222774–228777 | 6004 | 1287 | 429 | 4 | 3 | 5.48 | 48.37 |
| VviMAPKKK20 | GSVIVT01012686001 | CBI23211.3 | 10 | + | 641937–648890 | 6954 | 3369 | 1123 | 8 | 7 | 6.09 | 123.96 |
| VviMAPKKK21 | GSVIVT01012895001 | CBI25598.3 | 11 | + | 6576324–6581862 | 5539 | 1470 | 490 | 9 | 8 | 5.40 | 54.29 |
| VviMAPKKK22 | GSVIVT01015494001 | CBI28162.3 | 11 | − | 4194704–4202353 | 7650 | 3066 | 1022 | 7 | 6 | 8.36 | 114.09 |
| VviMAPKKK23 | GSVIVT01017915001 | CBI26208.3 | 5 | + | 4619229–4633132 | 13904 | 2061 | 687 | 17 | 16 | 6.76 | 75.81 |
| VviMAPKKK24 | GSVIVT01017968001 | CBI26245.3 | 5 | + | 5145293–5148759 | 3467 | 1632 | 544 | 11 | 10 | 5.43 | 63.24 |
| VviMAPKKK25 | GSVIVT01018020001 | CBI26291.3 | 5 | − | 5521761–5528578 | 6818 | 1371 | 457 | 7 | 6 | 8.32 | 51.27 |
| VviMAPKKK26 | GSVIVT01018052001 | CBI26318.3 | 5 | + | 5824109–5839208 | 15100 | 3354 | 1118 | 11 | 10 | 5.59 | 124.59 |
| VviMAPKKK27 | GSVIVT01019010001 | CBI17559.3 | 4 | + | 17821355–17829046 | 7692 | 906 | 302 | 5 | 4 | 6.66 | 33.93 |
| VviMAPKKK28 | GSVIVT01019630001 | CBI34567.3 | 2 | + | 2093246–2099598 | 6353 | 1791 | 597 | 16 | 15 | 6.59 | 68.4 |
| VviMAPKKK29 | GSVIVT01019739001 | CBI34657.3 | 2 | + | 2874833–2882840 | 8008 | 2706 | 902 | 11 | 10 | 9.12 | 97.91 |
| VviMAPKKK30 | GSVIVT01019821001 | CBI34722.3 | 2 | + | 3631707–3639546 | 7840 | 2088 | 696 | 15 | 14 | 6.37 | 78.14 |
| VviMAPKKK31 | GSVIVT01020712001 | CBI21988.3 | 12 | + | 2837402–2887099 | 49698 | 2454 | 818 | 11 | 10 | 5.99 | 91.70 |
| VviMAPKKK32 | GSVIVT01021854001 | CBI34208.3 | 14 | − | 6462432–6466040 | 3609 | 1821 | 607 | 10 | 9 | 5.22 | 69.40 |
| VviMAPKKK33 | GSVIVT01021884001 | CBI34231.3 | 14 | − | 6026347–6048725 | 22379 | 2631 | 877 | 16 | 15 | 5.50 | 97.14 |
| VviMAPKKK34 | GSVIVT01022098001 | CBI21399.3 | 7 | + | 16573422–16578723 | 5302 | 1287 | 429 | 8 | 7 | 5.31 | 47.06 |
| VviMAPKKK35 | GSVIVT01022115001 | CBI21414.3 | 7 | − | 16707463–16710545 | 3083 | 1233 | 411 | 8 | 7 | 4.66 | 44.78 |
| VviMAPKKK36 | GSVIVT01022116001 | CBI21415.3 | 7 | + | 16711209–16721450 | 10242 | 2469 | 823 | 7 | 6 | 8.72 | 89.41 |
| VviMAPKKK37 | GSVIVT01023037001 | CBI23895.3 | 12 | + | 16524280–16552240 | 27961 | 1296 | 432 | 11 | 10 | 7.05 | 48.78 |
| VviMAPKKK38 | GSVIVT01023048001 | CBI23901.3 | 12 | + | 16381443–16399280 | 178238 | 525 | 175 | 5 | 4 | 8.42 | 20.19 |
| VviMAPKKK39 | GSVIVT01023216001 | CBI29680.3 | 12 | + | 21019776–21020867 | 1092 | 1092 | 364 | 1 | 0 | 8.88 | 39.99 |
| VviMAPKKK40 | GSVIVT01023958001 | CBI37812.3 | 3 | − | 2141138–2162161 | 21024 | 1743 | 581 | 16 | 15 | 5.56 | 65.53 |
| VviMAPKKK41 | GSVIVT01024578001 | CBI15829.3 | 6 | − | 8664971–8669192 | 4222 | 1896 | 632 | 7 | 6 | 6.83 | 71.74 |
| VviMAPKKK42 | GSVIVT01026487001 | CBI37539.3 | 4 | + | 22814276–22820209 | 5934 | 1803 | 601 | 12 | 11 | 8.99 | 65.01 |
| VviMAPKKK43 | GSVIVT01026546001 | CBI37576.3 | 4 | + | 21993962–21997860 | 3899 | 1701 | 567 | 10 | 9 | 5.52 | 64.16 |
| VviMAPKKK44 | GSVIVT01027189001 | CBI40585.3 | 15 | − | 17151471–17154829 | 3359 | 1776 | 592 | 10 | 9 | 5.82 | 67.45 |
| VviMAPKKK45 | GSVIVT01028897001 | CBI22687.3 | 16 | − | 17707492–17719297 | 11806 | 2679 | 893 | 11 | 10 | 9.43 | 95.93 |
| VviMAPKKK46 | GSVIVT01029055001 | CBI33351.3 | 5 | + | 11545076–11551320 | 6345 | 2859 | 953 | 13 | 12 | 5.42 | 105.79 |
| VviMAPKKK47 | GSVIVT01029147001 | CBI17788.3 | 11 | + | 19186659–19204657 | 17999 | 2937 | 979 | 11 | 10 | 5.40 | 108.81 |
| VviMAPKKK48 | GSVIVT01029426001 | CBI35320.3 | 17 | + | 17077436–17089089 | 11654 | 555 | 185 | 4 | 3 | 5.62 | 21.36 |
| VviMAPKKK49 | GSVIVT01030044001 | CBI28411.3 | 12 | − | 9089290–9097431 | 8142 | 2496 | 832 | 8 | 7 | 8.80 | 93.29 |
| VviMAPKKK50 | GSVIVT01030194001 | CBI18047.3 | 8 | + | 10650328–10661181 | 10854 | 1014 | 338 | 6 | 5 | 8.85 | 37.96 |
| VviMAPKKK51 | GSVIVT01030202001 | CBI18051.3 | 8 | − | 10519384–10524077 | 4694 | 1881 | 627 | 7 | 6 | 5.29 | 71.71 |
| VviMAPKKK52 | GSVIVT01031721001 | CBI32391.3 | 3 | − | 3812818–3816504 | 3687 | 1194 | 398 | 8 | 7 | 8.32 | 43.57 |
| VviMAPKKK53 | GSVIVT01032232001 | CBI24046.3 | 11 | + | 13477774–13485357 | 7584 | 447 | 149 | 3 | 2 | 5.90 | 16.90 |
| VviMAPKKK54 | GSVIVT01032389001 | CBI34850.3 | 14 | − | 26886951–26893203 | 6253 | 1059 | 353 | 6 | 5 | 6.90 | 39.53 |
| VviMAPKKK55 | GSVIVT01032487001 | CBI34936.3 | 14 | + | 27812667–27819608 | 6942 | 1083 | 361 | 6 | 5 | 6.34 | 40.52 |
| VviMAPKKK56 | GSVIVT01033779001 | CBI30245.3 | 8 | − | 17884683–17902051 | 17369 | 2238 | 746 | 17 | 16 | 6.14 | 82.96 |
| VviMAPKKK57 | GSVIVT01034710001 | CBI40217.3 | 13 | − | 8150739–8202754 | 52016 | 2277 | 759 | 15 | 14 | 7.89 | 85.25 |
| VviMAPKKK58 | GSVIVT01034988001 | CBI22876.3 | 5 | − | 694778–701765 | 6988 | 2199 | 733 | 14 | 13 | 6.42 | 81.58 |
| VviMAPKKK59 | GSVIVT01035409001 | CBI20668.3 | 4 | + | 1079723–1090460 | 10738 | 2631 | 877 | 15 | 14 | 5.26 | 97.28 |
| VviMAPKKK60 | GSVIVT01036758001 | CBI24172.3 | 19 | − | 22924599–22935504 | 10906 | 3138 | 1046 | 10 | 9 | 5.23 | 115.67 |
| VviMAPKKK61 | GSVIVT01037773001 | CBI26734.3 | 19 | − | 7730105–7732118 | 2014 | 894 | 298 | 2 | 1 | 6.17 | 34.22 |
| VviMAPKKK62 | GSVIVT01038760001 | CBI32969.3 | 12 | + | 610174–641062 | 30889 | 1476 | 492 | 8 | 7 | 6.05 | 55.76 |
| VvMAPKKKKs | ||||||||||||
| VvMAP4K1 | GSVIVT01012233001 | CBI27303.3 | 5 | + | 7076729–7078360 | 1632 | 1362 | 454 | 4 | 3 | 5.20 | 88.93 |
| VvMAP4K2 | GSVIVT01013739001 | CBI28527.3 | 9 | − | 18395010–18410644 | 15635 | 1704 | 568 | 15 | 14 | 9.31 | 41.78 |
| VvMAP4K3 | GSVIVT01014297001 | CBI20268.3 | 1 | − | 8012586–8021182 | 8597 | 1107 | 369 | 9 | 8 | 6.34 | 78.12 |
| VvMAP4K4 | GSVIVT01016074001 | CBI25246.3 | 14 | − | 27646939–27657107 | 10169 | 1803 | 601 | 16 | 15 | 5.58 | 62.83 |
| VvMAP4K5 | GSVIVT01019643001 | CBI34578.3 | 2 | − | 2150787–2160118 | 9332 | 2190 | 730 | 22 | 21 | 6.68 | 81.13 |
| VvMAP4K6 | GSVIVT01027718001 | CBI23577.3 | 1 | − | 342583–373752 | 31170 | 2430 | 810 | 19 | 18 | 5.80 | 50.65 |
| VvMAP4K7 | GSVIVT01032461001 | CBI34913.3 | 19 | − | 2416077–2441445 | 25369 | 2121 | 707 | 20 | 19 | 5.81 | 67.08 |
The identified open reading frames (ORFs) are classified into four subfamilies, MAPK, MAPKK, MAPKKK, and MAPKKKK. Columns 1–13 contain the protein acronym (Name), coding sequence (CDS), Vitis proteome 12× ID, GenBank ID, chromosome location (Chr), strand (str), gene length, number of introns and exons, protein length, estimates of molecular weight, and isoelectric point of the protein (pI) for each gene are given.