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. 2010 Dec;23(6):595–608. doi: 10.1089/vim.2009.0113

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 4. — F3 from RV-infected cells inhibits T-cell function more than F3 from non-infected cells. CD4⁺ T cells purified by magnetic beads were stained with CFSE and simultaneously exposed to a polyclonal stimulus (SEB: SEB/α-CD49d/α-CD28), and F3 from mock-infected (SEB + Mock), or RRV-infected cells (SEB + RRV), or media. After 5 d the cells were stained with violet viability dye and anti-CD3 and 1 × 10⁵ cells were acquired and analyzed with a FACS Aria. (A) The percentages of viable cells are indicated in the upper panels, and the percentage of proliferating cells (CFSE) are indicated in the lower panels. (B) The percentages of viable cells and proliferating cells of 14 independent experiments using 7 different F3 preparations and lymphocytes from 8 different donors are shown, comparing 5 μg or equivalent quantities of AChE activity (mU) of MV mock and RRV (median 1.07 μg, range 0.6–1.3 of mock protein per 5 μg of RRV protein). Medians are depicted and significant differences between groups were established with the Wilcoxon or Mann-Whitney U test (*p < 0.05; **p < 0.0002).

FIG. 4. — F3 from RV-infected cells inhibits T-cell function more than F3 from non-infected cells. CD4⁺ T cells purified by magnetic beads were stained with CFSE and simultaneously exposed to a polyclonal stimulus (SEB: SEB/α-CD49d/α-CD28), and F3 from mock-infected (SEB + Mock), or RRV-infected cells (SEB + RRV), or media. After 5 d the cells were stained with violet viability dye and anti-CD3 and 1 × 10⁵ cells were acquired and analyzed with a FACS Aria. (A) The percentages of viable cells are indicated in the upper panels, and the percentage of proliferating cells (CFSE) are indicated in the lower panels. (B) The percentages of viable cells and proliferating cells of 14 independent experiments using 7 different F3 preparations and lymphocytes from 8 different donors are shown, comparing 5 μg or equivalent quantities of AChE activity (mU) of MV mock and RRV (median 1.07 μg, range 0.6–1.3 of mock protein per 5 μg of RRV protein). Medians are depicted and significant differences between groups were established with the Wilcoxon or Mann-Whitney U test (*p < 0.05; **p < 0.0002).