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. 2015 May 11;156(8):2905–2917. doi: 10.1210/en.2015-1074

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Figure 4. — The differing effects of chemical treatments on gene expression in organotypic hypothalamic slice cultures. A, qPCR analysis of AVP, hnAVP, OT, and hnOT in PVN after 24 hours of treatment with 10 μM forskolin compared with vehicle (DMSO) controls. B, Relative mRNA expression of Azin1, Odc, Az1, c-Fos, AVP, hnAVP, OT, and hnOT was investigated after 48 hours of treatment with 10 μM putrescine (Put), 5 mM DFMO, and 5 mM DFMO and 10 μM Put compared with vehicle controls. Values are means ± SEM of n = 5 animals per group. *, P < .05; **, P < .01; ***, P < .001. Con, control.