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. 2015 May 11;156(8):2905–2917. doi: 10.1210/en.2015-1074

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Figure 6. — Lentiviral-mediated manipulation of Azin1 and Odc expression in the SON alter AVP gene expression. A, Relative expression of Odc, c-Fos, AVP, hnAVP, OT, and hnOT in the SON of Azin1 shRNA compared with control shRNA in rats subjected to metabolic analysis of 7-day SL (control shRNA, n = 11 SON; Azin1 shRNA, n = 11 SON). Rats were unilaterally injected into SON with lentiviral vectors expressing rat Azin1 and GFP (B) or rat Odc and GFP (C), and the expression of Azin1, c-Fos, AVP, hnAVP, OT, and hnOT was investigated 2 weeks later (GFP, n = 4 SON; Azin1, n = 4 SON; Odc, n = 4 SON). Inset (B, C) are Western blots of AtT20 cells (Sigma) transduced (5 multiplicity of infection) for 48 hours with lentiviral vectors expressing Azin1 (B) or Odc (C) together with GFP controls. Values are means ± SEM. *, P < .05; **, P < .01; ***, P < .001.