Figure 5.

Body weight was affected by age, and timing and duration of E2 treatment. A, In MAT and AG rats monitored for 3 months, body weight was significantly higher in vehicle-treated rats compared with E2-treated rats of both ages. B, Effects of 6 months of continuous vehicle or E2, or switching treatment at the 3-month time point, are shown for the AG rats. Body weight was sensitive to E2 status even after a 3-month delay in hormone treatment. n = 10 rats per group. Letters at each time point refer to groups that are similar (same letter) or significantly different (different letter). Group numbers correspond to numbering in Figure 1. C, Repeated measures ANOVA within each group identified body weight changes over the 3- to 6-month period, with numbers referring to levels being similar to (same number) or significantly different from (different number) other time points within that same group. Numbers 1–4 also represent body weight from lowest (1) to highest (4) within a group.