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. 2015 Jun 3;4(8):932–944. doi: 10.5966/sctm.2014-0197

Figure 3.

Figure 3.

Histological analysis of induced pluripotent stem cell-derived neural cells 8 weeks after transplantation. (A): Immunofluorescence images of grafts for venus (green) and TH (magenta). Scale bar = 500 μm. (B–D): Quantitative analysis of the graft volume (B), the total number of TH-positive (TH+) neurons (C), and the number of TH+ neurons per graft volume (D). Each value is given as the mean ± SEM (n = 4–6). ∗, p < .05; ∗∗∗, p < .001. (E): Immunofluorescence images of the grafted neural cells for venus (green), GIRK2 (magenta), and TH (cyan). Scale bar = 50 μm. (F): Quantitative analysis of the ratio of GIRK2+/TH+ neurons in each graft. Each value is given as the mean ± SEM (n = 4–6) with no significant difference. (G): Immunofluorescence images of neural cells for venus (green), calbindin (magenta), and TH (cyan). Scale bar = 50 μm. (H): Quantitative analysis of the ratio of calbindin+/TH+ neurons in each graft. Each value is given as the mean ± SEM (n = 4–6) with no significant difference. Abbreviations: 1W, 1 week after administration; 8W, 8 weeks after administration.