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. 2015 Jun 10;4(8):913–926. doi: 10.5966/sctm.2015-0030

Figure 2.

Figure 2.

Functional analysis of bacterially injected TALENs. (A): Fluorescence intensities of control EB5 cells (EB5), EB5 cells transfected with eukaryotic expression plasmids encoding the TALENs (transfection), EB5 cells infected by TALEN1, or EB5 cells infected by TALEN1&2. The cells were analyzed by flow cytometry 3 days after transfection or injection. (B): A representative GFP-negative EB5 cell colony (arrow) 3 days after bacterial delivery of the gfp-targeting TALEN protein pair, observed under a fluorescence microscope. (C): Sequence alignment of the TALEN-targeting region among the GFP-negative EB5 cells after bacterial delivery of the gfp-targeting TALEN protein pair. Abbreviations: EB5, GFP-expressing B5 mouse embryonic stem cell line; GFP, green fluorescent protein; TALEN, transcription activator-like effector nuclease; TALEN1, PAK-J∆STY/pExoS54-FLAG-TALEN1; TALEN1&2, PAK-J∆STY/pExoS54-FLAG-TALEN1 and PAK-J∆STY/pExoS54-FLAG-TALEN2; WT, wild type.