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. 2015 Jun 10;4(8):913–926. doi: 10.5966/sctm.2015-0030

Figure 3.

Figure 3.

Factors influencing the bacterial delivery of TALEN proteins. (A): The number of green fluorescent protein-expressing B5 mouse embryonic stem cell line (EB5) cells surviving infection of PAK-J∆STY/pExoS54-FLAG-TALEN1 and PAK-J∆STY/pExoS54-FLAG-TALEN2 (1:1 ratio) at the indicated MOI for 3 hours. The number of surviving cells was compared with that of the no infection control using a two-sample t test. ∗, p < .05; ∗∗, p < .001; ∗∗∗, p < .0001. Error bars represent SD of triplicate assays. (B): TALEN proteins injected into EB5 cells after infection at the indicated MOI for 3 hours. Nuclear protein extracts from the same number of surviving cells were prepared and subjected to Western blot analysis by anti-FLAG antibody. (C): Fluorescent-activated cell sorting (FACS) analysis results of EB5 cells 3 days after TALEN injection at the indicated MOI for 3 hours. The FACS data of the infected cell populations were compared with those of the no injection control using a two-sample t test. ∗, p < .05; ∗∗, p < .001; ∗∗∗, p < .0001. Error bars represent SD of triplicate assays. Abbreviations: MOI, multiplicity of infection; TALEN, transcription activator-like effector nuclease.