Figure 4.
A) Box plot showing the number of CTBP2 puncta per inner hair cell in four regions of interest (1, 2, 3 and 4 mm from the apex) in three groups of mice, terminated at 5-7 months (15 mice), 22-24 months (26 mice) and 27-29 months (22 mice) of age respectively. The means at 22-24 months are significantly lower than those at 5-7 months in all four regions of interest (p <.01). The means at 27-29 months are not significantly different from those at 22-24 months. Boxes indicate 25th-75th percentile of data; bar indicates median; and whiskers indicate 5th-95th percentile of data. B) When the four regions of interest are combined there is a significant decrease in the overall mean number of connections at 22-24 and 27-29 months of age compared to 5-7 months of age (p <.01) Asterisks indicate significance (p<0.01). Significance was determined using ANOVA with a Bonferroni/Dunn Post-hoc test (Statview).