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. 2015 Apr 20;29(8):3238–3252. doi: 10.1096/fj.15-271395

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Reversible expression of BAT biomarker genes in RP, ING, and interscapular fat from adult mice maintained at 23°C or exposed to 4°C for 7 days. A) Experimental protocol. Expression profiles of Ucp1 mRNA and protein levels in 56 and 63D B6 (C57BL/6J) (B) and AxB8 (C) mice. Corresponding mRNA levels of Dio2, Pgc1a, and Ppara in 56 and 63D B6 (D) and AxB8 (E) mice (n = 7–10 mice/group). Data are expressed as means ± sem. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Significances are for the effects of rearing temperature.