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. 2015 Apr 24;29(8):3399–3410. doi: 10.1096/fj.14-269720

Figure 1.

Figure 1.

Efficient differentiation of hiPSCs into stable chondrocytes. A) Chondrogenic differentiation of hiPSCs. B) Representative morphologic changes in hiPSCs during chondrogenic differentiation at different stages (undifferentiated hiPSCs, d 7, 12, and 14 after differentiation). Scale bar, 100 μm. C) Representative immunofluorescence staining of chondrocyte markers, Sox9 and Col2a1 during hiPSC differentiation at d 4, 7, and 14. Scale bar, 100 μm. D) Gene expression analyses using quantitative real-time PCR for pluripotency markers (OCT4 and NANOG), mesodermal markers (KDR, Brachyury T, and THY-1), Sox trio chondrogenic markers (SOX9, SOX6, and SOX5), joint interzone-specific markers (WNT9A and SOSTDC1) and collagens and ECM markers (COL1A1, COL2A1, COL10A1, and ACAN). Data are expressed as means ± sd for gene expression vs. hiPSCs (logarithmic scale) and represent 3 independent experiments.