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(A) Principle of cell-based, fluorescence high-throughput screening assay. (B) Chemical structures of ANO1 inhibitors. (C) YFP fluorescence measured in single wells of 96-well plates, showing inhibitory effect of idebenone, miconazole and plumbagin on ANO1 channel activity. Indicated concentrations of idebenone, miconazole and plumbagin were added 20min prior to ANO1 activation by 100 μM ATP.
