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. 2015 Jul 1;192(1):30–39. doi: 10.1164/rccm.201412-2293OC

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Copyright © 2015 by the American Thoracic Society

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Figure 2. — Protein gene product 9.5 (PGP9.5) immunoreactivity in human airway epithelium. (A and B) PGP9.5-immunopositive (green, Alexa Fluor 488) varicose epithelial fibers (arrowheads) appeared in patches at the luminal edge of the epithelium. (C and D) A population of epithelial cells were immunoreactive for PGP9.5, with typical flask-shaped pulmonary neuroendocrine cell morphology (asterisks) and cellular projections (open arrows). Segments of epithelial fibers were also observed (arrowheads). (E and F) Epithelial fibers were mostly 200-kD neurofilament (NF200) immunonegative (open arrow), but some were observed only with antineurofilament (red, Alexa Fluor 568, solid arrow) or were dual positive (arrowheads). Biopsies were from right main bronchus (A, D, and E) and right middle lobe entrance (B, C, and F). Results shown are independent experiments of six biopsies from separate patients. Scale bars = 100 μm. Unprocessed, single-channel images, additional images, and videos are shown in Figures E4–E6 and Videos E2 and E3.