Figure 1. Notch mutant alleles block temperature-induced NMJ outgrowth.

A) Representative camera lucida examples of NMJ arbors from N^ts1 and N^ts2 alleles, muscle fibers 7 and 6, 13 and 12, and low and high temperatures. B) Quantification of NMJ complexity is shown as the average number of boutons and branches per arbor under the above experimental conditions. Bars in the top half represent the mean number of boutons, and in the bottom half, the number of branches. Error bars are +/− standard error of the mean. Horizontal lines at 100 boutons and 10 branches have been added for visual reference. P-values are given for the comparison between the adjacent 18°C and 29°C categories. For all figures, *** = p-value < 0.005, ** = 0.005 < p-value < 0.01, * = 0.01 < p-value < 0.05. The number of replicates per experiment is between 9 and 19 for all experiments in all figures unless otherwise noted. Bouton counts were not normalized for muscle fiber area, as the latter did not vary with statistical significance across experimental groups.