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. 2015 May 8;10:20. doi: 10.1186/s40793-015-0001-5

Table 3.

M2B3 Reporting Standard about a sample

  Descriptor name Description of usage Control vocabulary/format/unit Example
  SAMPLE_Title A short informative description of the sample. Must be unique for each sample, (i.e. for each filter generated during sampling). Format: <OSD_SiteID > _ < Month > _ < Year > _ < SiteName > _ < Protocol_Label > _ < SampleNo > _ < Depth> OSD3_06_14_Helgoland_NPL022_1_surface
  SAMPLE_Depth The distance below the surface of the water at which a measurement was made or a sample was collected. Format: ##.#; 1.5
Positive below the sea surface.
SDN:P06:46:ULAA for m
SAMPLE_Protocol_Label Identifies the protocol used to produce the sample, e.g. filtration and preservation. Term list; NPL022
See the SAMPLE_Protocol_Label in the OSD Protocols Section for details
SAMPLE_Quantity Refers to the quantity of environment that was sampled, most often with dimensions Length, Amount, Mass or Time. Format : ###.### 20 Litres
See the SAMPLE_Quantity in the OSD Protocols Section for details
SAMPLE_Container Refers to the container in which the sample is stored prior to analysis. Term list; Sterivex cartridge
See the SAMPLE_Container in the OSD Protocols Section for details
SAMPLE_Content Refers to the content of the sample container. While the sample might target a specific organism (e.g. bacteria), the sample content might be a filter or a volume of water. Term list; Particulate matter on a 0.22 μm pore size filter
See the SAMPLE_Content in the OSD Protocols Section for details
SAMPLE_Size-Fraction_Upper-Threshold Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Term list; no pre-filtration
See the SAMPLE_Size-Fraction_Upper-Threshold in the OSD Protocols Section for details; in μm
SAMPLE_Size-Fraction_Lower-Threshold Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are exclude from the sample. Term list; 0.22
See the SAMPLE_Size-Fraction_Lower-Threshold in the OSD Protocols Section for details; in μm
SAMPLE_Treatment_Chemicals Refers to the chemicals (e.g. preservatives) added to the sample. Terms list: ChEBI; None
See the SAMPLE_Treatment_Chemicals in the OSD Protocols Section for details
SAMPLE_Treatment_Storage Refers to the conditions in which the sample is stored, e.g. temperature, light conditions, time. Term list; −80 degrees Celsius
See the SAMPLE_Treatment_Storage in the OSD Protocols Section for details

Mandatory information is in bold and other fields are recommended. OSD Protocols are available at http://www.microb3.eu/sites/default/files/osd/OSD_Handbook_v2.0.pdf. ChEBI is an ontological classification and dictionary of small chemical compounds (http://www.ebi.ac.uk/chebi/init.do).

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