Fig. 5.

Schematic representation of the time course of Sirt2, FoxO1a, SOD-2 formation as well as of intracellular ROS levels during adipocyte differentiation in 3T3-L1 murine preadipocytes. The transient increase in intracellular ROS levels is shut down through induction of FoxO1a target genes such as SOD-2. Following a brief period of Akt-dependent phosphorylation of FoxO1a (p-FoxO1a, referring to FoxO phosphorylated at Ser253 – the equivalent of human FoxO1a Ser256), it is upregulated during adipogenesis, and it becomes transcriptionally active after the clonal expansion phase due to its deacetylation through interaction with Sirt2 [106,135,171,222].