Fig. 2.

Reduction of intracellular superoxide levels in cells treated with tempol, Gentian Violet or Brilliant Green. In (A), MCF-7 cells were left untreated or treated with Tempol (T, 5 mM), Gentian Violet (GV, 5 µM) or Brilliant Green (BG, 5 µM). In (B), ZR75.1 cells were left untreated or treated with Tempol (3 mM), Gentian Violet (5 µM) or Brilliant Green (5 µM). Intracellular superoxide levels were monitored using staining with the fluorescent dye DHE. Fluorescence (ex: 488 nm/em: 575 nm) was acquired by flow cytometry and the median of fluorescence was quantified. Values are means±SEM from n≥3. Statistical comparison (treated cells vs untreated cells) was performed using a t-test.