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Tempol, Gentian Violet and Brillant Green treatment impair cyclin D1 expression. MCF-7 (A) and ZR75.1 (B) were left untreated or treated for 24 h with Tempol (T), Gentian Violet (GV) or Brilliant Green (BG) at the indicated concentrations. WCE were resolved by SDS-PAGE and analyzed by immunoblot using anti-cyclin D1 and anti-actin antibodies. Experiments were performed three times. Representative immunoblots are shown.
