Fig. 2.

H&E staining (A and B) did not reveal any grossly abnormal structures, lipid accumulation, or inflammation. As observed by PAS staining, the glomerular basement membranes (blue arrows) and the muscular layer of the afferent arterioles (yellow arrows) of (C) WT and (D) EtOH fed mice were of similar thicknesses. Immunohistochemical analysis of kidney tissue demonstrates a major increase in Cyp2E1 protein levels between control (E) and ethanol feeding groups (F) and minor increase in 4-hydroxynonenal-protein adducts (G and H) as a result of ethanol metabolism (400× magnification). [P=proximal convoluted tubule, D=distal convoluted tubule, G=glomerulus, size bar=20 μm (C and D) and 50 μm (A, B, E, F, G and H).]