Fig. 3.

Effect of GSNO treatment and radiation on STAT3 activities and HNSCC cell survival. (A) Following the treatment of SCC1, SCC14a and SCC22a HNSCC cell lines with 10 Gy radiation and 250 μM GSNO for 24 h, the levels of STAT3 and their phosphorylated forms were analyzed by Western analysis using specific antibodies. The densitometry values as compared to that of control calculated by using NIH-Image-J. (B) HNSCC cell lines were treated with 10 Gy radiation and 250 μM GSNO for indicated time and the cell survival was analyzed as described under Section 2. The results in bar and line graphs were given as mean of at least three independent experiments. The vertical lines indicate the standard deviation; *P<0.05; **P<0.01; compared with vehicle (VHC; dimethylsulfoxide) treated control groups.