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. 2015 Feb 27;10:23. doi: 10.1186/s40793-015-0013-1

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© McIlroy et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Maximum-likelihood phylogenetic tree of 16S rRNA genes for all Meganema isolates and closely related species in the LTP database (LTPs111) [ 10 ] constructed using the ARB software [ 13 ]. The 1342 bp long sequence fragment of the unique 16S rRNA gene copy in the genome is identical with the previously published 16S rRNA gene sequence for the Gr1^T strain (AF18048). A 20% maximum frequency filter was applied in order to remove hypervariable positions. Included are all uncultured clone sequences from the NCBI database which share ≥94% sequence similarity with the Gr1^T strain (all were ≥ 98%), with sequences from the same study clustered at ≥ 99% similarity and a representative included. Bootstrap values, calculated from 100 re-samplings, are indicated for branches with > 50% support. Scale bar represents substitutions per nucleotide. The family Fusobacteriaceae was used as the out-group.